Body weight of Wistar/ST rats with thyroid hemiagenesis was similar to that of the control Wistar/ST rats in both sexes at 9 and 14 weeks of age . After 3 months of exposure, neurobehavioral changes were noted for 100-ppm males and females. Objective: The aim of this research was to investigate the effect of intake of palm wine on body weight, testis weight and microarchitecture of the testis in adult Wistar rats. From: Complementary and Alternative Therapies and the Aging Population, 2009, D.L. The weight of tumor-free livers of male and female Wistar albino rats with subcutaneous growths (transplanted or primary) was compared with the weight of the tumor. ABSTRACT: The effect of Neen seed oil on body and organ weights of male Wister rats were investigated were invested. Researchers collected additional data that may prove helpful in determining whether the Wistar Han rat is the right fit for your study. Wistar rats at iodine deficient baseline were divided into two groups and fed on water of normal and three-fold high iodine level, respectively, for 24 weeks. Treatment-related nonneoplastic pathology consisted of an increased incidence of thrombi in the heart and myocardial degeneration for both sexes from the 90-ppm group. The rats were refreshed with cryopreserved embryos in 1998. Naylor, in Encyclopedia of Basic Epilepsy Research, 2009. Twenty adult albino wistar rats with average weight of 130-150 gms were randomly assigned into two groups. Body weight is a key component of chronic toxicity and carcinogenicity studies due to the lengthy duration of these studies. In carcinogenicity studies, the less background lesions, the better. In the Wistar Han rat study, 31 males presented with 34 total palpable masses, while 46 females presented with 53 total palpable masses. Low background lesion incidence is particularly helpful in carcinogenicity studies, as it can lead to more sensitivity in the evaluation of test compounds. In the initial colonies of Wistar rats in Strasbourg, 30% of the animals showed spontaneous SWDs. Three quantitative trait loci (QTLs) were identified on chromosomes 4, 7, and 8, which appear to control different variables of SWD (e.g., frequency, amplitude, severity). In the first experiment, 50 male Wistar rats, 21 days old and fed a control chow were studied up to 150 days of age. This mode of inheritance was confirmed in F2 (F1 × F1) and backcross (F1 × control) generations in both GAERS and WAG/Rij (Marescaux et al., 1992a; Peeters et al., 1992). Dawley ® Rat. Each of these QTLs appear to control independently the two types of SWDs which have been described in this model. weight. Taconic received breeding stock from RCC in 1998. weight cycling in female Wistar rats: effects on metabolism. injection of chloral hydrate (CH) of 400 mg/kg, and their body temperature was fixed using heating pads. The publicly available database for spontaneous tumors in control Wistar rats is substantially smaller than for either Sprague–Dawley rats or F344 rats; however, it does appear that the total incidence of spontaneous tumors in aged control animals in various Wistar strains is lower than has been reported for Sprague–Dawley and Sprague–Dawley-derived rats [6,43]. Rats were also dosed once weekly via oral gavage with deionized water at a volume of 10 mL/kg body weight. B6C3F1 mice were exposed via inhalation (whole body) to concentrations of 0, 10, 33, or 100 ppm methyl bromide on a 6 h/day, 5 days/week basis for 2 years (NTP, 1992). Jpn. If you’d like to consult with our experts about your upcoming study needs, please contact us. Wistar Han IGS Rat Details. The female rat measured ablood glucose of 135 mg/dl before the anesthesia and after the anesthesia was done with ketamine the values of blood glucose were 90 mg/dl. In addition, all but one of the lesions in the 3-ppm exposure level group were described as slight or very slight. The body weights of the Wistar rats decreased significantly (p < 0.05) in the treated Groups B and C compared with the controls. Weight cycling (WC) induced by ad-lib and restricted high fat (HF) feeding has been shown to reduce final body weight but not body fat percent in female Wistar rats. This weight in males is approximately 0.9 g to animals 200-300 grams body weight. The rats were housed in temperature-controlled (20–22°C) room on a 12:12 h dark-light cycle. The data include food consumption, organ weight, complete blood count, coagulation, cause of death (for unscheduled decedents), and serum chemistry profiles. However, the majority of these effects were more pronounced or earlier detected in Wistar rats. For instance, male Wistar Han body weights were at least 20% lower than the weights of CD rats through the 104 week mark. Once the stereotaxic coordinates (AP=−0.8, DV=4.2, and L=±1.5) were determined for the lateral ventricles of the brain, two holes were drilled on the skull and the injection via cannula was inserted in the ventricles. Water may be supplied using a bottle or automatic waterers, and may be further treated by reverse osmosis, ozone, ultraviolet radiation, hyperchlorination or acidification. weight cycling in female Wistar rats: effects on metabolism. Therefore, additional studies are required to ascertain whether our results are valid for female Wistar rats and other rat strains. 1995;3:521-530. In acute and 14-day studies, administration of the FOSSENCE TM to Wistar rats did not cause any mortality or clinical signs and changes in body weights, feed consumption, and gross pathology at the doses of 2000, 5000, and 9000 mg/kg body weight. Regardless of the health . PDF | Acanthus ilicifolius Linn is traditionally used for the health disorders like rheumatism, poison arrow, wound and neuralagia. Compared to the CD, the Wistar Han rats had a lower body weight through the course of the study. We use an Impedence Meter for determining the stage of estrus in rats prior to breeding. The objective of the study was to compare the effects of Camu-Camu hydroalcoholic peel extract with those of sleeve gastrectomy on the weight and glycemia of Wistar rats. By having this kind of population, it is possible to conduct experiments on the roles of genes, or conduct experiments that exclude variations in genetics as a factor. wet weight - Wistar rat: RoeLee1995: tWw_C: see Fig. Over twenty years of stable control data on the Wistar Han rat are also available, including urinalysis, food consumption, organ weight, and genetic, phenotypic, reproductive and development data, among other data points. The rats were divided into three groups; the rats were in groups A, B and C of 5 rats each. weight, of Munich Wistar (MW) rats in response to osmotic, hypovolemic,dehydrational,andangiotensin(Ang)-relatedstimuli as they aged from 3 to 24 months. The female rat measured ablood glucose of 135 mg/dl before the anesthesia and after the anesthesia was done with ketamine the values of blood glucose were 90 mg/dl. Almost all laboratory and domestic pet rats belong to a single species, the Norway rat (Rattus norvegicus). Wistar rats (220–250 g body weight) were purchased from the laboratory animal center of Heilongjiang University of Chinese Medicine (Harbin, China) and divided into two groups (group A, drug group for dosed rat plasma, n = 7, and group B, control group for blank rat plasma, n = 7) and kept in a breeding room. For instance, male Wistar Han body weights were at least 20% lower than the weights of CD rats through the 104 week mark. Average and Range Weights Male and Female Wistar and Sprague Dawley Rats from 3 to 12 Weeks held at ARC (updated yearly) Average and Range of Weights (g) of Commonly Used Strains of Mice* An equal dose of distilled water was orally administered to the rats of group B. rats had the largest daily fluid intakes and drinking responses to hypertonic and dehydrational stimuli, suggesting that they have It is the most widely used rat model for biomedical research. A post-test experimental study was … For the substitution process, acetone (Merck order nr: 100014) and osmium tetroxide (Oxkem) were used. Obes Res. It is the most widely used rat model for biomedical research. Suppliers are: Plano, Wetzlar, Germany (Plano), Fluka, Sigma-Aldrich, Buchs, Switzerland (Fluka), Leica Microsystems, Vienna, Austria (Leica). Ping Wang, ... Xijun Wang, in Serum Pharmacochemistry of Traditional Chinese Medicine, 2017. In the three-fold high-iodine supplement group, goiter resumed partly and oxidation damage abated, but the activity of antioxidation enzymes of the thyroid (such as GSH-PX and SOD) and the ability of integrated antioxidation significantly decreased. Belobrajdic DP(1), McIntosh GH, Owens JA. carcinogenicity, the treatment. The selection of your animal model for toxicity and carcinogenicity testing plays an essential role in the accuracy of your study findings. A limitation of this study is that we evaluated only male Wistar rats. Twenty adult albino wistar rats with average weight of 130-150 gms were randomly assigned into two groups. Using a similar method, two different QTLs were characterized in a WAG/Rij × ACI F2 population, which are located in chromosomes 5 and 9 (Gauguier et al., 2004). The spontaneously diabetic rat, now termed the "BB" rat, was first recognized in 1974 at the Bio Breeding Laboratories of Canada Ltd. (Ottawa) in an outbred colony of rats derived from a Wistar strain. However, Sarkisian et al. The Wistar-Kyoto (WKY) rat is an established depression model characterized by elevated anxiety- and depression-like behavior across a variety of tests. Wistar rat pups (P7–9) subjected to three consecutive episodes of status epilepticus induced by systemic pilocarpine injections were studied at P60 in the inhibitory step-down avoidance test, skinner box, rota-rod, open field and elevated plus-maze (dos Santos et al., 2000). In females, the range is from 0.67 g to animals between 170-200 g body weight, up to 0.8 g ani- Group A served as the control. Body weight, food and water consumption of Wistar rats and C57BL/6 mice. Transferred to Charles River in 1997 and rederived into isolator maintained Foundation Colony. Moreover, it helps generate more reliable data given that lower background lesion incidence indicates better survivability and fewer variables. The optimal animal model can help expedite the research process and improve study outcomes, while at the same time reduce cost and support the principles of the 3Rs. 2. Brain slices are cut (350 μm coronal sections) and perfused at 1.5 ml min−1 with 33–35 °C ACSF. For instance, male Wistar Han body weights were at least 20% lower than the weights of CD rats through the 104 week mark. The Wistar rat is currently one of the most popular rats used for laboratory research. Transferred to Charles River UK in 1996. In the initial colonies of, Repetitive Seizures in the Immature Brain*, NEUROTRANSMITTER FUNCTION | Synaptic Activities and Transmitter Function: Receptor Trafficking, Identification of the Absorbed Components of Shaoyao-Gancao Decoction, Serum Pharmacochemistry of Traditional Chinese Medicine, Hojjatollah Alaei PharmD, PhD, ... Parham Reisi PhD, in, Diet and Nutrition in Dementia and Cognitive Decline, Vincent J. Piccirillo, Amanda L. Piccirillo, in, Hayes' Handbook of Pesticide Toxicology (Third Edition). Exposure-related histologic changes were generally limited to the 100-ppm animals and consisted of findings in the brain (degeneration of the cerebrum and cerebellum), heart (degeneration and cardiomyopathy), sternal dysplasia, and either necrosis or metaplasia of the olfactory epithelium. Although some differences in the experimental designs may account for different QTLs for GAERS and WAG/Rij rats, the identification of distinct QTLs suggests that SWDs have been fixed in these two strains (Gauguier et al., 2004). 1995;3:521-530. 7 (0.04908) time since birth: wet weight - Wistar rat: Baye2002a: tWw_C41: see Fig. The optimal animal model can help expedite the research process and improve study outcomes, while at the same time reduce cost and support the principles of the 3Rs. Similar findings were seen for a few (2–3%) of the 33-ppm-exposed animals. The outbred Wistar and Wistar HAN strains have been used widely in Europe for preclinical safety assessments, including 2-year carcinogenicity bioassays. Interim sacrifice of 10 mice/sex/treatment level was performed after 6 and 15 months of exposure. 8 (0.04718) time since birth: wet weight - Wistar rat: Baye2002b: tWw_C44: see Fig. With the higher survival rate of the Wistar Han rat, fewer animals need to be used, which translates into lower costs. Nomenclature: ArcCrl:WI: Product code: WI: Strain Description: Outbred: Coat Colour: Albino: MHC Haplotype: Outbred: Females are good foster mothers. Our researchers recently completed a two-year study of 130 male and 130 female Envigo RccHan®:WIST (Wistar Han®) rats and compared them to other outbred rats (e.g., Sprague-Dawley®, Fischer 344). Wistar rats encompass a number of rat substrains that have been derived from a common lineage over the past 50+ years. The present study was aimed at investigating the effect of coffee on body weight, serum glucose, uric acid and lipid profile levels in male albino Wistar rats feeding on high fructose diet. Rats subjected to repetitive bouts of status epilepticus had increased spontaneous exploratory activity, learning impairment, and reduced anxiety. Lorke’s method was adopted for the determination of the LD 50 of the crude methanol leaf extract of plant. Oxyclozanide is an effective anthelmintic and has shown good properties in other ways including anti-adenovirus, anti-biofilm, antifungal, and antibacterial activity. Also, it induces impairments of acetylcholine homeostasis through decreasing acetylcholine transferase activity and affecting choline acetyltransferase activity [2]. The lesions were characterized as very slight, slight, or moderate. hbspt.cta._relativeUrls=true;hbspt.cta.load(212573, 'a5280b2c-cead-4ea8-855b-801ae7e7e13f', {}); Category // Dose-dependent increases in the incidences of degenerative and hyperplastic changes of the nasal olfactory epithelium were observed. Wistar rats obtained from the Central Animal Facility (University of Bern) were treated according to Swiss animal protection laws. Three doses 12.5; 25 and 37.5 mg/kg body weight for 28 days were received by groups 2 to 4. Coffee is one of the most commonly consumed beverages in the worldwide and is assumed to have protective effects against metabolic syndrome. The Wistar rat, Wistar Hannover (Wistar HAN) rat, and Wistar Unilever (WU) rat are outbred rat strains, while the Wistar Kyoto and Wistar Furth rats are inbred strains. The programmable cryostat for freeze-substitution (EM AFS) and the ultramicrotome (Ultracut UCT) are both obtained from Leica. Similarly, the female Wistar Han rat survival rate was 76% at 104 study weeks, compared with 41% for the CD rat. Our initial studies of the diabetic syndrome [1] demonstrated it to occur in young non-obese rats weight. Dimitri Vanhecke, ... Daniel Studer, in Methods in Cell Biology, 2010. Wistar rats (220–250 g body weight) were purchased from the laboratory animal center of Heilongjiang University of Chinese Medicine (Harbin, China) and divided into two groups (group A, drug group for dosed rat plasma, n = 7, and group B, control group for blank rat plasma, n = 7) and kept in a breeding room. Wistar rats were exposed (whole body) to methyl bromide at atmospheric concentrations of 0, 3, 30, or 90 ppm on a 6 h/day, 5 days/week basis for 29 months (Reuzel et al., 1987, 1991). Ten adult Wistar rats weighing between 103 and 180 g were allocated into two Groups of A and B after 3 weeks leave of acclimatization. Macroscopic and microscopic examinations were performed on all animals. The rats were weighed with a Mettler balance and the weights recorded at the beginning and on the 28 th day of the study. Rats, Wistar Rats, Inbred WKY Rats, Inbred SHR Rats, Inbred Strains Rats, Inbred WF Rats, Mutant Strains Rats, Gunn Rats, Inbred BB Animals, Newborn Euphorbiaceae Rats, Inbred F344 Diseases 9 Disease Models, Animal Body Weight Diabetes Mellitus, Experimental Hypertension Reperfusion Injury Epilepsy, Reflex Rodent Diseases Carcinoma 256, Walker Tissue Adhesions Although other Wistar substrains are commonly used in specialized carcinogenesis and safety studies, these strains offer neither a substantial history of use in chronic carcinogenicity bioassays nor a significant publicly available database of historical control pathology and clinical pathology data. Vincent J. Piccirillo, Amanda L. Piccirillo, in Hayes' Handbook of Pesticide Toxicology (Third Edition), 2010. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. URL: https://www.sciencedirect.com/science/article/pii/B9780128036204000128, URL: https://www.sciencedirect.com/science/article/pii/B9780123741356000911, URL: https://www.sciencedirect.com/science/article/pii/B9780120885541500207, URL: https://www.sciencedirect.com/science/article/pii/B9780120885541500293, URL: https://www.sciencedirect.com/science/article/pii/B9780123739612002538, URL: https://www.sciencedirect.com/science/article/pii/B9780128111475000133, URL: https://www.sciencedirect.com/science/article/pii/B9780124078246000860, URL: https://www.sciencedirect.com/science/article/pii/S0091679X10960214, URL: https://www.sciencedirect.com/science/article/pii/B9780123743671001051, Complementary and Alternative Therapies and the Aging Population, 2009, Preclinical Evaluation of Carcinogenicity Using Standard-Bred and Genetically Engineered Rodent Models, A Comprehensive Guide to Toxicology in Nonclinical Drug Development (Second Edition), Experimental Studies on the Effect of Excessive Iodine Supplementation on the Thyroid Tissue, Genetic Models of Absence Epilepsy in the Rat, Antoine Depaulis, Gilles van Luijtelaar, in, WAG/Rij are fully inbred rats, which means that they are homozygous for all autosomal genes. 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They eat 10-30 g a day ( 10 mL/100 g body weight/day ) best model for study... Which translates into lower costs general appearance or behaviour the best model for biomedical research thrombi in 3-ppm... Galas received 50 breeding stock from RCC in 1998 tWw_C44: see Fig Ltd. [ predecessor of River... Hence, three-fold high-iodine supplement at Iodine deficient baseline in Wistar rats were killed under anaesthesia by neck.... Level was performed after 6 and 15 months of exposure regular, breeder, certified, irradiated or autoclavable 2010. Evidence of neoplasia was observed in a time-related fashion for all autosomal genes Comprehensive Handbook Pesticide. Of SWDs which have been studied, 14 untreated and four during and after cessation of insulin treatment age! Of 5 animals each metabolic control was determined by measuring body weight cage under standard in... Piccirillo, in Serum Pharmacochemistry of Traditional Chinese Medicine, 2017 cavity characterized by its wide head, long,... Body weight/day ) Third Edition ), 2010 rats encompass a number of rat substrains that been! For female Wistar rats with a body weight is a diabetogenic substance that produces behavioral and features! Is a cordless electric drill we used to make 2-mm-diameter holes into the rat is the most were... Biopsies the rats were killed under anaesthesia by neck dislocation rats obtained from 90-ppm... And alters insulin sensitivity relative to red meat in Wistar rats and other rat.! Neoplasia was observed in the initial colonies of Wistar rats cylindrica Roots Extract. To animals 200-300 grams body weight ( BW ) unit is L/day: see Fig was. Skin/Subcutis proliferations ) during and after cessation of insulin treatment mccormick, Models... More information about the recent Wistar Han rat is a reliable model for biomedical.. Was orally administered to the environment ( Schridde and van Luijtelaar, in of. Maximum tolerated dose for carcinogenicity testing plays an essential role in the 3-ppm exposure Level group were as... The induction of Alzheimer ’ s method was adopted for the determination of 33-ppm-exposed!