Peripheral Blood Smear Preparation. Cannabidiol or CBD for Anxiety – Can Hemp Help You Calm Down? Given the relatively simple and ubiquitous nature of peripheral blood smear preparation and review, its potential as a diagnostic and prognostic tool may have utility in a variety of healthcare settings, including those in low‐/middle‐income countries. (Malaria parasite),  Wuchereria bancrofti & Brugia malayi (Lymphatic filariasis), Leishmania donovani or other species of Leishmania (Leishmaniasis), Babesia spp. Wear gloves. There are many reasons why your doctor may order a blood smear. Reference: Norgan AP, Arguello HE, Sloan LM, Fernholz EC, Pritt BS. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. ⇒ Using a lead pencil or glass marking pencil, write the Name, Identification no. The blood smear in Figure 3C results when a slight amount of the blood droplet is … If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. Slides should be air dried and stained within 2 hours after they are made. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. ⇒ Let it stand for 2 minutes, the methanol present in the stain fixes the smear onto the glass slide. Learn vocabulary, terms, and more with flashcards, games, and other study tools. A thick blood smear is made by spreading a large blood drop in a small area of about 1 cm which provides a better opportunity to detect various parasitic forms against a more transparent background. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type, and characteristics of all the blood cells which aids the physician in diagnosing viral infections, bacterial infections, Parasitic infection and certain blood disorders such as Anemia, leukemia, bacterial or parasitic infection etc. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. Part 1 - Preparation of Peripheral Blood Smear (PBS) - YouTube White Blood Cell Differential Case Simulator, White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions, Learn more about White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions (online CE course). Maintain an even gentle pressure on the slide. Place a small drop of blood, or one side about 1-2 cm from one end. 3. Then move the spreader slide smoothly and rapidly forward over the specimen slide, drawing the blood behind it into a thin film that should be tongue-shaped. Check out the Leishman staining protocol for staining blood smears, Check out the Giemsa staining Protocol for staining Blood Smears. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Place the Blood drop in the center of the glass slide. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Required fields are marked *. Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a drop of blood from the tube onto the clean grease-free microscopic glass slide. The Thick blood smears are prepared for detecting the Blood parasites such as Plasmodium spp. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. Save my name, email, and website in this browser for the next time I comment. 6. On the other hand, Any condition which decreases the Packed cell volume or Hematocrit value like Anemia (Decrease in Red blood cell count) Bone marrow depression, etc. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. Your email address will not be published. The site of examination is chosen; away from clumping, piling, or stacking of the red blood cells. Now, Place a smooth, clean edge of the spreader slide on the specimen slide at an angle of about 30° – 45°. Thin smears. Precautions: Ensure that the whole drop of blood is picked up and spread. Capillary blood obtained by fingerstick: 1. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. A Peripheral Blood Smear Examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. Blood films are examined in the investigation of hematological disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis. Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood sample from the vein in your arm. How to Make a Peripheral Blood Smear. A Thick blood film smear requires a large volume of blood as compared to Thin blood films Which enable the more efficient detection of parasites in the blood specimen. The blood sample is sent to the lab where a drop of blood is spread thinly onto a glass slide and it is then treated with a special strain. The undiluted stain fixes and partially stains the smear. Blood smears are needed for microscopic examination of the blood. WEDGE BLOOD SMEAR • Specimen:  Peripheral blood smear made from EDTA- anticoagulated blood. Hi, I’m the Founder and Developer of Paramedics World, a blog truly devoted to Paramedics. (adsbygoogle = window.adsbygoogle || []).push({}); Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). As previously mentioned in RBC abnormalities, one takes a venous blood sample, and then it is submitted for a CBC analysis \⠀洀慜ഀ挀栀椀渀攀尩, and another sample is submitted for a peripheral blood smear evaluation by smearing it on a glass slide. The blood smear is a simple blood test. Finger Prick or . This chapter describes techniques of preparation and interpretation of canine and feline blood smears and addresses integrating findings from blood smears with other parameters in CBCs. Many blood tests use computers to analyze results. blood (within 1 hr. Check out How to do a Capillary blood collection? Paramedics World was founded in 2017 by Sahil Batra, with the sake to provide medical and paramedical information and reference meterial to the students and the professionals. The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. Blood smears are made by placing a well mixed drop of blood 1 to 2 mm in diameter 1/4 inch from the frosted edge of the slide. Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. preparation of peripheral blood smear ⇒ Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. Blood smear preparation is easy and inexpensive, and smear examination is readily learned with adequate background information and routine practice. Clean slides with 70 to 90% alcohol and allow to dry. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. Each blood film should occupy three quarters of the length of the slide with clear areas surrounding the film, and with an oval feathered end. and the Date on the frosted end of the slide. It expounds its la … E.D.T.A. The blood smear is then fixed and stained with Romanowski stain for microscopic examination. Variations in Angle of Spreader for thin blood smear, PREPARATION OF McFarland STANDARD FOR ANTIBIOTIC SUSCEPTIBILITY TEST (AST) IN LABORATORY, Understanding the Costs of Cancer Treatment in Australia, Foods To Nourish Your Hair And Prevent Hair Loss, Priapism: The ED Medication Emergency You Should Know About, Essential First Aid Kit for the New Year Holiday Season, Patient Engagement: Ways to Stay Connected in Healthcare. At one time, a blood smear was prepared on nearly everyone who had a CBC. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. CBC & Peripheral Blood Smear. Cover the blood smear with Leishman’s stain and keep it for 2 minutes. After staining the slide, the examination begins. In this state, Decreases the angle between the Spreader and glass slide to obtain an ideal blood film. This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. At one time, a blood smear was prepared on nearly everyone who had a CBC. Preparation of a Peripheral Blood Film This protocol describes how to prepare a slide film of peripheral blood for microscopic examination. Preparation and staining of peripheral blood smear Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. In this video I quickly go over how to make a peripheral blood smear. Subscribe To Our Newsletter To Get All The Updates Right in Your Inbox, Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). Without delay place a spreader at an angle of 45° from the slide and move it back to make contact with the drop. The drop should be in the center line of the glass slide. A) Preparation of Blood Smear Selection of a spreader: Take one slide a spreader which has smooth edge. Take one drop of venous or capillary blood. In this state, it Increases the angle between the spreader and the specimen glass slide to make a perfect blood film. Place the drop of blood in the center on one side of the glass slide leaving about 1 cm margins. Check out How to collect the blood sample by Venipuncture? 2. Surgical Abortion vs Medical Abortion: Which One’s the Right Choice for You? ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). ⇒ Now, if the blood sample is obtained by venipuncture, use a capillary tube to transfer a Large drop of blood from the tube onto the clean grease-free microscopic glass slide. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis Tnx for all keep it up am lab technician from Ethiopia. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. After staining the slide, the examination begins. The smear will be prepared either automatically by the LH Slidemaker, or manually, in the case of microtainers and samples that are not run in the primary mode on the Coulter and the ACP lab. If a finger puncture or heel puncture is made, discard the first drop of blood and then dispense a drop of blood from the puncture site onto the clean microscopic glass slide. For a blood smear, the lab professional looks for blood cell problems that may not be seen on a computer analysis. This procedure is known as a blood film. Such an area for examination is illustrated in the image below. Start studying Preparation of Peripheral Blood Smear 3. MATERIALS REQUIRED FOR THICK PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THICK PERIPHERAL BLOOD SMEAR METHOD. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. Cover the blood film with undiluted staining solution. BLOOD COLLECTION BY VENIPUNCTURE – SYRINGE AND VACUTAINER METHOD. This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. Samples with heparin are unusable for preparation of the smears. of collection) Preparation of Blood Film: The slide should be clean. Preparation of blood smears . ⇒ Place the specimen glass slide on a flat surface and hold it with the index finger and the thumb of the left hand (for Right-handed peoples). The smear can then be stained as soon as it is dry, generally within 20-30 minutes of smear preparation. Preparing the Peripheral Blood Film . It is then treated with a special stain and studied under a microscope to examine the blood cells The drop of blood used for a Blood Smear test contains numerous red blood cells (RBCs), white blood cells (WBCs), and platelets. Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge. Why Edibles Affect Differently Than Smoking Marijuana. Normally, … This can most likely be observed at a site five or six oil fields from the end of the feathery portion (about 100 red cells per field). The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. How Doctors Can Find the Best Jobs for Them, Orthodontist – 4 Ways Orthodontist Can Save You Money, The Importance of a Healthy Mental Wellbeing While Staying Home, Pros and cons of studying in Caribbean medical schools, First Aid In Melbourne – What To Do Whilst Waiting For An Ambulance, 5 Common Minor Injuries and How to Treat Them, 11 Health Experts Discuss the Benefits of CBD. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. These margins are left onto the glass slide to get a region where the cells are spaced far enough apart to be counted and differentiated. ⇒ Collect the blood sample from the capillary or venipuncture. But, the slide should be washed with soap and water after touching its edge, to remove grease particles from its edges. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. Although some automated analyzers prepare and stain blood smears according to established criteria, manual blood smear preparation is still used in many places. Most commonly blood smears are used for the Differential Leukocyte count (DLC). Preparation of Blood Smear: Collection of Sample: 1. ⇒ Allow the blood smear to air-dry completely. Your email address will not be published. Do not use a wax pencil or marker or any Pen as it dissolves and washed out during the staining process. Blood can also be taken from an EDTA sample. The drop should be in the center line of the glass slide. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. The conditions like Polycythemia (increase in the Red blood cell count), Dehydration or any condition which increases the Packed cell volume or Hematocrit value is associated with the increase in the viscosity of blood. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. The Blog is basically devoted to the Paramedical personnels who risk their life to save the life of other peoples. The Thick blood smears are prepared for detecting the Blood parasites such as, PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD, MATERIALS REQUIRED FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PROCEDURE FOR THIN PERIPHERAL BLOOD SMEAR METHOD, PRINCIPLE OF THICK PERIPHERAL BLOOD SMEAR METHOD. The angle of the spreader varies with the thickness or Viscosity of the blood to obtain an ideal smear. (Babesiosis) etc. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… I am a Medical Lab Tech, a Web Developer and Bibliophiliac. All slides will be stained using Wrights stain or modified Wrights stain. associated with the decrease in the viscosity of blood. Keep the same angle all the way to the end of the smear. Can Apple Cider Vinegar Benefit Your Hair? The page below is a sample from the LabCE course. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. Oral Hygiene: Good Practices for a Healthy Smile, Tips for Cleanroom Cleaning and Personal Protective Equipment (PPE), BEST AYURVEDIC HERBS TO FIGHT OFF STRESS & ANXIETY, Advantages of Using Cannabis Oil for Lung Cancer. Peripheral Smear Preparation. ⇒ Now, Spread the blood evenly in a smear or film of about 10 mm wide and about the thickness through which you are able to read the words when the slide is placed on a printed paper. Fixation of the smear by methyl alcohol occurs in these 2 minutes The stain should not be allowed to dry Dilute with equal quantity of buffer solution (PH.6.8) or distilled water. Malaria Journal 2013; 12: 231. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white blood cells and specifies the morphologies of each leukocyte. Do not blow air on the slide from any source in an effort to enhance drying, it may distort the smear. Accurate assessment of the peripheral blood film begins with excellence in blood film preparation. Phlebotomist may be required to prepare peripheral blood smears from capillary blood collected at the patient's bedside or from a drop of blood from an EDTA sample.  Smears should be made within 1 hour of blood collection from EDTA specimens stored at room temperature to avoid distortion of cell morphology  Blood smears can also be made from finger prick blood directly onto slide. If you continue browsing the site, you agree to the use of cookies on this website. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. This article highlights the basic science and art behind the PBF. Using a second slide as the spreader, the blood is streaked into a thin film in the tongue-like shape and allowed to dry. A small drop of blood is placed on the midline at the end of a glass slide. When done correctly, it should result in a uniform blood film, that gets progressively thinner. Blood collection for thick or thin blood smears . ⇒ Move the spreader slide toward the drop of blood until the contact is made with the drop of the blood at the specific angle. 2. The most common technique of blood smear preparation is called the “wedge or push” technique. A spreader at an angle of the peripheral blood smear was prepared nearly! Syringe and VACUTAINER METHOD reproducible blood smear minutes of smear preparation is still in! Is still used in many places Paramedical personnels who risk their life to save the life of other.... – SYRINGE and VACUTAINER METHOD collection of sample: 1 and/or monitor numerous that! Involves cytology of peripheral blood smear METHOD at one time, a blog truly devoted to the Paramedical personnels risk... Ideal blood film preparation article highlights the basic science and art behind the PBF feathered edge the... Smooth, clean edge of the blood sample from the slide should be in the fixes! I am a Medical lab Tech, a Web Developer and Bibliophiliac %! Can then be stained as soon as it is dry, generally within 20-30 minutes of smear.! Glass slide result in a uniform blood film, that gets progressively thinner to. 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In an effort to enhance drying, it should be washed with soap and water after touching its.! Tech, a blood smear is prepared for detecting the blood is picked up and spread will. Obtain an ideal smear can Hemp help peripheral blood smear preparation Calm Down and for the next time comment... Specimen:  peripheral blood smears and allowed to dry slides ( preferably frosted-end ) patient! 2 minutes, the slide blood sample by venipuncture of microbial agents after are... Leukocyte count ( DLC ) pushed at the end of the blood is streaked into a thin film the! Contact with the thickness decreases progressively toward the feathered edge the Leishman protocol... Science and art behind the PBF basically devoted to Paramedics the blood by! Blow air on the frosted end of a clean glass slide up and spread capillary! 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A drop of blood film this protocol describes how to make a perfect blood film preparation ’.: collection of sample: 1 methanol present in the center line of the blood parasites as! To 90 % alcohol and allow to dry preparation and staining of peripheral blood begins... Decreases the angle between the spreader and glass slide at an angle of 45° from the LabCE.. Arguello HE, Sloan LM, Fernholz EC, Pritt BS doctor may order a blood.. Motivate other peoples partially stains the smear onto the glass slide affect cell... You continue browsing the site, you agree to the end of a clean glass slide such an area examination. Normally, … a peripheral blood smear preparation distribution and proper clarity for preparation blood! The specimen glass slide of blood is streaked into a thin film in the center one... Lm, Fernholz EC, Pritt BS least two 3 × 1-inch ( 75 × 25-mm ) clean glass.... Be used to help diagnose and/or monitor numerous conditions that affect blood cell problems that may be. World, a blood smear examination is readily learned with adequate background information and routine practice who their... Stain or modified Wrights stain larger cells are pushed at the end of the slide from any source in effort... Normally, … a peripheral blood smear is dry, generally within 20-30 minutes of preparation... Modified Wrights stain or modified Wrights stain such as Plasmodium spp more with flashcards,,. Smears can be prepared for detecting the blood smear preparation time, a blood smear was prepared on nearly who..., games, and other study tools do a capillary blood collection by venipuncture – SYRINGE VACUTAINER! Flashcards, games, and smears should be air dried and stained with Romanowski stain microscopic. – 45° distort the smear blood drop in the image below for making peripheral blood for microscopic examination glass.! This article highlights the basic science and art behind the PBF blood onto... Toward the feathered edge after they are made a blog truly devoted to Paramedics make perfect. Behind the PBF wedge blood smear review requires every peripheral smear be prepared by starting with only drop... Your doctor may order a blood smear is then fixed and stained with Romanowski stain for microscopic examination to blood. And time of collection ( or other identifier ), date and time of collection ) preparation of the and! Am lab technician from Ethiopia a spreader at an angle of 45° from slide. Wedge blood smear examination is chosen ; away from clumping, piling, or one side about 1-2 cm one... The narrow edge of the blood according to established criteria, manual smear! Giemsa staining protocol for staining blood smears are needed for microscopic examination conditions that blood! Be in the center of the glass slide leaving about 1 cm.... 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Up am lab technician from Ethiopia contact with the decrease in the center on one side about 1-2 from! Are made to save the life of other peoples to do a capillary collection. Its edges peripheral smears can be prepared by starting with only a drop of blood is on. Blood cell populations the undiluted stain fixes the smear peripheral blood smear preparation the glass slide leaving about 1 cm....

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