Loss of ATP-citrate lyase results in severe developmental effects, with the production of asexual spores (conidia) being greatly reduced and a complete absence of sexual development. Western blot analysis of ATP citrate lyase using A) 30 µg PC-12 whole cell lysate and B) 30 µg Rat2 whole cell lysate. Furthermore, Remmers et al. TATA (1994) 302, 759-764 (Printed in Great Britain) Organization of the 5' region of the rat ATP citrate lyase gene Kyung-Sup KIM,* Sahng-Wook PARK, Young-Ah MOON and Yoon-Soo KIM Department of Biochemistry and The Institute of Genetic Science, Yonsei University College of Medicine, 134 Shinchon-Dong, Seodaemun-Ku, Seoul 120-752, Korea Agenomic clone, encompassing the 5' … ATP-citrate (pro-S-)-lyase. ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. A definitive role for ATP citrate lyase in tumorigenesis has emerged from ATP citrate lyase RNAi and chemical inhibitor studies, showing that ATP citrate lyase inhibition limits tumor cell proliferation and survival and induces differentiation in vitro. promoter region of ATP citrate lyase (ACLY) gene in Murrah buffalo (Bubalusbubalis) and native Vechur cattle. The 4 substrates of this enzyme are ADP, phosphate, acetyl-CoA, and oxaloacetate, whereas its 3 products are ATP, citrate, and CoA.. ATP citrate lyase (ACLY) is an enzyme that in animals represents an important step in fatty acid biosynthesis. Acetyl CoA is a vital building block for the endogenous biosynthesis of fatty acids and cholesterol and is involved in isoprenoid-based protein modifications. In enzymology, an ATP citrate synthase (EC 2.3.3.8) is an enzyme that catalyzes the chemical reaction. The enzyme of C.limicola (Cl-ACL) was encoded in two adjacent open reading frames, aclB (1197 bp) and aclA (1827bp), whose products showed significant similarity to the N- and C-terminal regions of the human enzyme, respectively. PCR amplification of the promoter region generated a fragment of 768 bp in both Buffalo and Vechur cattle.Sequence analysis revealed a few elements/consensus sequences, which have potential role in regulation of transcription. Introduction ATP-citrate lyase (ACL) (EC 4.1.3.8) catalyzes the forma- tion of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phos- phate. They predicted, therefore, that the homologous gene in the human would be located on chromosome 17, probably close to PPY which is situated at 17q22-q24. ATP citrate-lyase gene; Response region; Insulin; Polyunsaturated fatty acids I. Has a central role in de novo lipid synthesis. ATP citrate lyase (ACLY), an important enzyme involved in lipid biogenesis linked with glucose metabolism, catalyzes the conversion of citrate to oxaloacetic acid (OAA) and acetyl-CoA. In the absence of ACLY, cells upregulate ACSS2 and utilize exogenous acetate to provide acetyl-CoA for de novo lipogenesis (DNL) and histone acetylation. In the cellular study, following small interfering RNA–mediated inhibition of ATP citrate lyase in MCF-7 cells, cell viability and apoptosis were measured using the Cell Counting Kit-8 and flow cytometry, respectively. Southern-blot analysis showed that the ATP citrate lyase gene exists as a single copy. Eur J Biochem. Select the link destinations: ... Gene expression databases. [2] By converting citrate to acetyl-CoA, the enzyme links carbohydrate metabolism, which yields citrate as an intermediate, with fatty acid biosynthesis, which consumes acetyl-CoA. ATP‐citrate lyase (ACLY) was initially identified as a ‘citrate cleavage enzyme’ in 1959, converting citrate into acetyl CoA and oxaloacetate . Histone acetylation in single-cell eukaryotes relies on acetyl coenzyme A (acetyl-CoA) synthetase enzymes that use acetate to produce acetyl-CoA. ATP citrate lyase/ACLY cDNA clones are full length sequence confirmed and expression validated. (1992) showed that in the rat the gene for ATP citrate lyase is closely linked to the gene for PPY, which in turn is close to the GH gene, on chromosome 10. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (Paraffin) (IHC (P)) applications. The gene encoding ATP-citrate lyase, designated ACLY, was mapped to human chromosome 17q12-q21 by PCR on a panel of human/rodent somatic cell hybrids and localized to 17q21.1 by PCR on a panel of radiation hybrids. 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